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Highly sensitive assay for g-glutamyltranspeptidase activity by HPLC with electrochemical detection
Summary
Type:
HPLC
Application #:
107670
Catalog#:
720018.40 - MN HPLC Column, Nucleosil C18, 100A, 7um, 4x250mm
Detection:
electrochemical, potential set to +0.55V versus Ag/AgCl
Conditions:
eluent: 0.1M Na phosphate buffer pH 3.5 containing 10μM EDTA - methanol (94:6, v/v)
flow rate 0.9ml/min
Authors:
Kiuchi K
Source:
J Chromatogr 357 (1986) 191 - 198
Details
Legend: Fig. 1. HPLC of A) mixture of standards in 0.01M hydrochloric acid (12.5pmol each of 1. dopa, 2. a-methyldopa (Mdopa) and 3. g-Glu-dopa), B) eluate from an aluminium oxide column, when 12.5pmol each
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